Development of Real Time RT-PCR Assays for Detection of Type A Influenza Virus and for Subtyping of Avian H5 and H7 Hemagglutinin Subtypes
Identifieur interne : 001186 ( Main/Exploration ); précédent : 001185; suivant : 001187Development of Real Time RT-PCR Assays for Detection of Type A Influenza Virus and for Subtyping of Avian H5 and H7 Hemagglutinin Subtypes
Auteurs : Francesca Sidoti [Italie] ; Francesca Rizzo [Italie] ; Cristina Costa [Italie] ; Sara Astegiano [Italie] ; Antonio Curtoni [Italie] ; Maria Lucia Mandola [Italie] ; Rossana Cavallo [Italie] ; Massimiliano Bergallo [Italie]Source :
- Molecular Biotechnology [ 1073-6085 ] ; 2010-01-01.
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Abstract
Abstract: Rapid detection and subtyping of H5 and H7 subtypes influenza A viruses are important for disease control in poultry and potential transmission to humans. Currently, virus isolation and subsequent HA and NA subtyping constitute the standard for avian influenza viruses detection and subtype identification. These methods are highly accurate and sensitive but are also laborious and time-consuming. Reverse transcription PCR and real time reverse transcription PCR assays, suitable tests for rapid detection, have previously been used for the specific diagnosis of H5 and H7 viruses, however, at present, no primer and probe sets are available for the identification of all H5 and H7 strains. Herein, we have developed specific and sensitive real time reverse transcription PCR assays for the detection of type A influenza virus and for subtyping of avian H5 and H7 hemagglutinin subtypes and we have also compared these molecular assays with viral isolation in terms of sensitivity. Our results demonstrate that the real time reverse transcription PCR assays are more sensitive, specific, less expensive compared to viral isolation. In conclusion, molecular assays could represent an useful tool for rapid detection and screening of H5 and H7 isolates during influenza A virus outbreaks alternatively to viral isolation.
Url:
DOI: 10.1007/s12033-009-9211-7
Affiliations:
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Inc</publisher><pubPlace>New York</pubPlace><date type="published" when="2010-01-01">2010-01-01</date><biblScope unit="volume">44</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="41">41</biblScope><biblScope unit="page" to="50">50</biblScope></imprint><idno type="ISSN">1073-6085</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1073-6085</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Avian influenza virus</term><term>H5 subtype</term><term>H7 subtype</term><term>Real time reverse transcription-PCR</term><term>Viral isolation</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Rapid detection and subtyping of H5 and H7 subtypes influenza A viruses are important for disease control in poultry and potential transmission to humans. Currently, virus isolation and subsequent HA and NA subtyping constitute the standard for avian influenza viruses detection and subtype identification. These methods are highly accurate and sensitive but are also laborious and time-consuming. Reverse transcription PCR and real time reverse transcription PCR assays, suitable tests for rapid detection, have previously been used for the specific diagnosis of H5 and H7 viruses, however, at present, no primer and probe sets are available for the identification of all H5 and H7 strains. Herein, we have developed specific and sensitive real time reverse transcription PCR assays for the detection of type A influenza virus and for subtyping of avian H5 and H7 hemagglutinin subtypes and we have also compared these molecular assays with viral isolation in terms of sensitivity. Our results demonstrate that the real time reverse transcription PCR assays are more sensitive, specific, less expensive compared to viral isolation. In conclusion, molecular assays could represent an useful tool for rapid detection and screening of H5 and H7 isolates during influenza A virus outbreaks alternatively to viral isolation.</div></front></TEI><affiliations><list><country><li>Italie</li></country><region><li>Piémont</li></region><settlement><li>Turin</li></settlement></list><tree><country name="Italie"><region name="Piémont"><name sortKey="Sidoti, Francesca" sort="Sidoti, Francesca" uniqKey="Sidoti F" first="Francesca" last="Sidoti">Francesca Sidoti</name></region><name sortKey="Astegiano, Sara" sort="Astegiano, Sara" uniqKey="Astegiano S" first="Sara" last="Astegiano">Sara Astegiano</name><name sortKey="Bergallo, Massimiliano" sort="Bergallo, Massimiliano" uniqKey="Bergallo M" first="Massimiliano" last="Bergallo">Massimiliano Bergallo</name><name sortKey="Cavallo, Rossana" sort="Cavallo, Rossana" uniqKey="Cavallo R" first="Rossana" last="Cavallo">Rossana Cavallo</name><name sortKey="Costa, Cristina" sort="Costa, Cristina" uniqKey="Costa C" first="Cristina" last="Costa">Cristina Costa</name><name sortKey="Costa, Cristina" sort="Costa, Cristina" uniqKey="Costa C" first="Cristina" last="Costa">Cristina Costa</name><name sortKey="Curtoni, Antonio" sort="Curtoni, Antonio" uniqKey="Curtoni A" first="Antonio" last="Curtoni">Antonio Curtoni</name><name sortKey="Mandola, Maria Lucia" sort="Mandola, Maria Lucia" uniqKey="Mandola M" first="Maria Lucia" last="Mandola">Maria Lucia Mandola</name><name sortKey="Rizzo, Francesca" sort="Rizzo, Francesca" uniqKey="Rizzo F" first="Francesca" last="Rizzo">Francesca Rizzo</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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